Figure 2
From: Using ex vivo arterial injection and dissection to assess white matter vascularization
Schematic representation of a simultaneous injection apparatus. It consisted of two reservoirs. The bigger (Res. 1) was a cylindrical glass aquarium accommodating the floating brain. The smaller (Res. 2) was a paraffin mounting bath containing glass gas washing bottles. In both reservoirs, the water temperature was 40 °C to prevent the gelatin solution from early solidification. To maintain the desired water temperature in the left tank, we used a low-temperature cooking machine with a built-in thermostat which simultaneously agitated/heated the water; the smaller reservoir had a built-in heating element and thermostat. The gas washing bottles were connected with silicon tubes to the catheters on one side (the anterior (a) and middle (b) cerebral arteries through the Y-connectors, the basilar artery (c)—directly) and a hand pump with a manometer on the other.
