Figure 3
From: Using ex vivo arterial injection and dissection to assess white matter vascularization
Technique of fiber dissection with preservation of the white matter vasculature. (A.1): Parenchyma and vessels before dissection. The pia mater covers both the cortex and subpial vessels, to which it is firmly attached. (A.2): Removal of the leptomeninges for classical dissection results in vessel avulsion, which hinders the study of white matter vasculature. (A.3): Sectioning of the superficial-most millimeters of gyri enables vessel preservation. (B.1): Classical fiber dissection with no vessels left. (B.2): Fibravasc technique. Vessels slip through the white matter layers removed and keep to be anchored to the deeper ones. If needed, vessels can be easily divided in-between the two layers. (C): Orthogonal view of the dissection step 1 (corresponds to the illustration (A.3)). (D): Orthogonal view of the dissection step 8 (corresponds to the (B.2)). (E): Cropped and enlarged picture contains the corona radiata, external capsule, and claustrum (from top to bottom) and presents vessels protruding from the white matter, as shown in (B.2). In (C,D and E), preserved vessels expose arterial distribution territories (blue—the anterior, red—medial, green—posterior cerebral artery). arach arachnoid mater, cl claustrum, cr corona radiate, cs central sulcus, cx cortex, dwm deep white matter, exc external capsule, ifof inferior fronto-occipital fasciculus, ilf inferior longitudinal fasciculus, ipl inferior parietal lobule, lf lateral fissure, mfg medial frontal gyrus, mtg medial temporal gyrus, pm pia mater, po pars orbitalis, pv pial vessel, sp spatula, uf U-fibers, unf uncinate fasciculus.
