Figure 4
From: Using ex vivo arterial injection and dissection to assess white matter vascularization
Injection results and 3D-printed holder. (A): Right lateral and (B): Anterior-inferior views of a human brain injected with colored gelatin (ACAs—blue, MCAs—red, PCAs—green). Pial arteries were filled with the pattern expected from the literature, while the superficial SupMCV remained collapsed. (C): Three pictures were taken from different angles to maximize the captured surface: oblique superior (left), orthogonal (middle), and oblique inferior (right). Light gray 3D-printed polylactic holder was filled with black paraffin to fix the position of the specimen. Although the relative position of the holder and cameras was maintained constant by a rigid support, additional fiducial markers (light pink circles) further improved the alignment of different steps images. Reference gray (RGB: 113, 113, 113) color patches (orange squares) are present to correct white balance and exposure precisely. ACA anterior cerebral artery, MCA medial cerebral artery, PCA posterior cerebral artery, SupMCV superficial middle cerebral vein.
