Figure 2
From: Wound healing approach based on excretory-secretory product and lysate of liver flukes
General wound healing evaluation. (A) Cytokeratin 19 (Krt19) gene expression normalized to Gapdh gene expression. V vehicle, ESPwe excretory-secretory product without endotoxin 10 µg, Lys 10 Lysate 10 µg, Us unwounded healthy skin. Data are presented as mean ± SEM, *p ≤ 0.05 as compared to unwounded skin, #p ≤ 0.05 as compared to a vehicle; (B) Infiltrate area, mm2. V vehicle, Chg chlorhexidine, BSA bovine serum albumin, ESP excretory secretory product 10 µg, ESPwe excretory secretory product without endotoxin 10 µg, Lys 10 Lysate 10 µg, Lys 50 Lysate 50 µg. Data are presented as mean ± SEM, *p < 0.05 as compared to a vehicle; (C) Wound area, hematoxylin and eosin staining, 10 days after treatment. The dotted line marked the wound area; (D) Arg1 gene expression normalized to Gapdh gene expression; (E) Nos2 gene expression normalized to Gapdh gene expression; (F) Leukotriene receptor B4 (Lblt4l) gene expression normalized to Gapdh gene expression; (D–F) Data are presented as mean ± SEM, *p ≤ 0.05 as compared to unwounded healthy skin, #p ≤ 0.05 as compared to a vehicle.
