Figure 8

Mortality of SeMNPV and microcapsule formulations following UVB treatment for 2 h, in 2nd instar larvae. The powders of SeMNPV OBs, CA-NPV, WP@CA-NPV, CS@CA-NPV and PDA@CA-NPV were evenly and thinly spread on weighing paper and exposed for 2 h to UVB output at a distance of 31 cm. The irradiated samples were added to the surface of food, and the 6-day-old second-instar larvae were placed into 24-well plates, one hole and one strip, and each sample was performed in triplicate. A small number of deaths occurred in each treatment on the third day after exposure, with mortality rates of 8.3% and 9.7% in the SeMNPV and PDA@CA-NPV treated groups, respectively. On day 5, a large number of deaths occurred, with 50% mortality being observed in the SeMNPV infected group. Also on day 5, the mortality of PDA@CA-NPV treated insects was 91.7%. Over time, on the 8th day, PDA@CA-NPV produced 100% mortality. The mortality of WP@CA-NPV and CS@CA-NPV groups was 95.8% and 88.9%, respectively. The mortality of SeMNPV infected larvae reached to 70.8%.