Figure 3
Detection of urinary platelets in podocyte injured mice. (A) Urinary protein and occult blood were detected by urine test strip in the urine 5 days after LMB2 injection [LMB2 (+)]. Those reactions were negative for LMB2-untreated mice [LMB2(−)]. Blood changes white paper to green, and protein changes yellow paper to green. Note that normal mice show 1 + proteinuria due to high concentration of small molecular weight proteins. (B) Confocal microscopy of Cy3-CD41 platelets in the urine. Immunostaining for CD41 showed platelets (arrows) were detected in the urine 5 days after LMB2 injection. Scale bar: 20 μm. (C) Confocal microscopy of DiI-platelets in the urine. DiI labeled platelets (arrows) were detected in the urine of mice injected with DiI platelets 5 days after LMB2 injection. Scale bar: 20 μm. Positive and negative control images for (B) and (C) are shown in supplementary Fig. 9.
