Figure 2 | Scientific Reports

Figure 2

From: Transcriptome-wide analysis reveals the coregulation of RNA-binding proteins and alternative splicing genes in the development of atherosclerosis

Figure 2

AS deregulation in atherosclerotic plaques in the early disease stage (SAMP_DIT) and advanced disease stages (SAMP_advanced). (A) Classification of all RAS events except IR events. X-axis: RAS event number. (B) Principal component analysis (PCA) of 18 SAMP_advanced and SAMP_DIT samples based on the percent spliced in (PSI) value of all differential nonintron retention (NIR) events. The samples were grouped by left and right atrial appendages and atherosclerotic stage, and the ellipse for each group is the confidence ellipse. (C) PSI heatmap showing all NIR-regulated alternative splicing events (RASEs) among SAMP_advanced and SAMP_DIT samples. NIR RASEs were clustered on the basis of K means. AS filtering was performed to detect splice junctions. AS is indicated if 80% or more of the samples contain 10-splice junction reads. (D) A GO analysis of RASGs in the SAMP_ advanced compared with the SAMP_DIT. E. Venn diagram showing the RASGs and differentially expressed genes (DEGs), p-value = 1.

Back to article page