Figure 5
Ileal tissue from inflamed pediatric CD patients vs. non-inflamed have distinct metabolic signatures. (A) A dendrogram depicting relative abundances of the 50 most significant compounds (top to bottom) compared between inflamed ileal tissue (red, left) and non-inflamed ileal tissue (green, right) patients (left to right). Data was obtained with reverse phase liquid chromatography mass spectrometry (LC–MS) in positive ion mode. Detailed LC–MS compound information is detailed in Supplemental Table 2. Supplemental Table 2 clarifies the y-axis labels, which are the feature numbers, the compounds, their elemental formulas, their molecular weight, as their chromatographic retention time. (B) Pathway analysis of pilot data using the mummichog analytic approach showed the enrichment of multiple metabolites including vitamin B3 metabolism, pyrimidine metabolism, nitrogen metabolism and other lipid and amino acid pathways in CD compared to control tissue groups, in alignment with our in silico findings.
