Table1 Optimization study.

From: Diarylation of thiazolopyrimidines by laccase and their in vitro evaluation as antitumor agents

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Entrya

Enzyme (ml)

Buffer

Co-solvent

Time(h)

Yieldb (%)

1

0.5

Phosphate buffer pH 8 (10 mM)

CH3CN

4

23

2

1

Phosphate buffer pH 8 (10 mM)

CH3CN

4

65

3

2

Phosphate buffer pH 8 (10 mM)

CH3CN

4

63

4

0

Phosphate buffer pH 8 (10 mM)

CH3CN

24

N.Rc

5

1

Phosphate buffer pH 8 (10 mM)

MeOH

24

N.R

6

1

Phosphate buffer pH 8 (10 mM

EtOH

24

N.R

7

1

Citrate buffer pH 3.5 (10 mM)

CH3CN

24

trace

8

1

Citrate buffer pH 3.5 (10 mM)

MeOH

24

N.R

9

1

Phosphate buffer pH 4.5 (10 mM)

MeOH

24

N.R

10

1

Phosphate buffer pH 7 (10 mM)

CH3CN

24

8

11

1

Phosphate buffer pH 4.5 (10 mM)

CH3CN

24

trace

  1. aAll reactions were performed using 2a (0.5 mmol), 3a (1 mmol) at ambient temperature. b Isolated yields. cNR: no reaction.
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