Figure 5
From: In-silico study of asymmetric remodeling of tumors in response to external biochemical stimuli
EZ-seed culture plate (a-1) and the schematic of the microwells (a-2) are shown. GB tumoroids were generated by seeding single cell suspension in the microwell array. The schematic of open-surface tumoroid-on-a-chip model that induces gradient of biochemicals is shown in (b-1). The chip consists of a central chamber for the GB tumoroid-embedded CH connected to a sink and a source reservoirs. To generate high and low gradients, short (b-2) and long (b-3) channels were designed to vary the diffusion length. Diffusion of FITC through the CH in channels was imaged, (c-1) and (c-2), and measured over time, (d), indicating lower diffusion (higher gradient) in long channels and higher diffusion (lower gradient) in short channels.
