Figure 2
UBQLN KO leads to pS129 accumulation while UBQLN2 overexpression decreases levels of pS129 in vitro. (A) Diagram of UBQLN2 and UBQLN1 protein structure. (B) Representative western blot of UBQLN 1,2, 4 Total Knock out (TKO) cells transfected to express WT α-syn alone or together with UBQLN1 (UB1) or UBQLN2 (UB2). Total α-syn and pS129 levels are decreased by co-expression of UBQLN2 but not UBQLN1. The ratio of pS129/total Syn is increased in both UBQLN1 and UBQLN2 co-expression (n = 8). (C) Representative western blot of TKO, UBQLN1 rescue, UBQLN2 rescue, and control cells were transfected with α-syn showing elevated pS129 levels in TKO cells compared to control cells, which is partially reversed by inducible co-expression of UBQLN2 but not UBQLN1. The ratio of pS129/total Syn is significantly increased in TKO compared to control and not reversed by UBQLN1 or UBQLN2 rescue (n = 8). Western blots were cropped to focus on protein(s) of interest. Uncropped Western blots are shown in Supplemental Figure S2.
