Figure 5
pS129 α-syn accumulates in A53T mice lacking UBQLN2. (A) Representative western blot of pS129 in RIPA-soluble brain lysates from the indicated transgenic mouse crosses. (B) Quantification from A showing accumulation of pS129 in A53TxUb2-KO mice (n = 6) and unchanged in A53TxUb2-hi mice (n = 7) compared to A53T controls (n = 8). (C) Ratio of pS129 to total Syn (see Fig. 4) does not differ significantly between groups (n = 8). (D) Representative images of pS129 expression in the cortex of A53T (n = 3), A53TxUb2-hi (n = 3) and A53TxUb2-KO (n = 3) show increased pS129 expression in A53T mice lacking UBQLN2 (12-month-old mice). (E) Representative western blots of endogenous pS129 in UBQLN2 transgenic mice. (F) Quantification of E showing a trend toward decreased endogenous murine pS129 levels in Ub2-hi mice (n = 4). (G) Analysis of the ratio of endogenous pS129 to total endogenous Syn (obtained from Fig. 4) shows no difference between groups (n = 4). Western blots were cropped to focus on protein(s) of interest. Uncropped Western blots are shown in Supplemental Figure S4.
