Figure 1 | Scientific Reports

Figure 1

From: An intranasal recombinant NDV-BRSV Fopt vaccine is safe and reduces lesion severity in a colostrum-deprived calf model of RSV infection

Figure 1

Construction of a recombinant NDV expressing a codon-optimized bovine RSV F protein. A functional transcription unit was inserted in the pNDV (LaSota strain) rescue plasmid at a unique restriction site (SacII) between the genes P and M. The additional transcription unit contains: NDV regulatory sequences Gene end-Intergenic-Gene Start (GE + IG + GS), Kozak sequence (K), a codon optimized synthetic sequence encoding the ectodomain of the bovine RSV F protein fused to the transmembrane and cytoplasmic domains (TM) of the NDV F protein.

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