Figure 5
Reduced viral burden in the lungs of rNDV-BRSV Fopt vaccinated colostrum-deprived calves. Treatment groups of calves included: unvaccinated, uninfected controls (n = 5); intranasal (i.n.) allantoic fluid vaccinated (mock vaccine control) and BRSV challenged (n = 6); i.n. wtNDV-vaccinated and BRSV challenged (n = 6); i.n. rNDV-BRSV Fopt vaccinated and BRSV challenged (n = 6). Lung homogenates were prepared using tissue collected at necropsy and a 10% suspension used to inoculate BT cells for virus isolation. Daily observations for cytopathic effect were conducted and recorded for 7 days following inoculation of cells. Results shown are the percentages of calves in each group which were positive for virus isolation. Statistical analysis on percentage data was performed using Kruskal–Wallis ANOVA.
