Figure 7
CXC chemokine gene expression in lung tissues of colostrum-deprived calves following BRSV experimental infection. Treatment groups of calves included: unvaccinated, uninfected controls (n = 5); intranasal (i.n.) allantoic fluid vaccinated (mock vaccine control) and BRSV challenged (n = 6); i.n. wtNDV-vaccinated and BRSV challenged (n = 5); i.n. rNDV-BRSV Fopt vaccinated and BRSV challenged (n = 6). Samples from lesioned and non-lesioned portions of lung were collected from calves 7 post-challenge with BRSV and placed in RNALater. The amount of each target gene was determined by quantitative real-time PCR with normalization to RPS9. Relative gene expression of each target was determined using the 2-ΔΔCt method. ΔΔCt values ± SE were transformed (2-ΔΔCt) and data are presented as the target gene expression relative to control lung samples (n = 4). Statistical analysis was performed using ANOVA. *p < .05, **p < .01.
