Figure 4 | Scientific Reports

Figure 4

From: GTS-21, a selective alpha7 nicotinic acetylcholine receptor agonist, ameliorates diabetic nephropathy in Leprdb/db mice

Figure 4

GTS-21 attenuates mitochondrial dysfunction and renal apoptosis. The db/db mice were treated with GTS-21 (4 mg/kg, IP, B.I.D.) or vehicle for 8 weeks. Western blot was used to measure apoptotic proteins (Cytochrome C in A, BAX in B, and cleaved caspase-3 in C). Densitometry data for the individual proteins was normalized to β-actin and is presented as relative densitometry units (RDU). Histological sections were immunofluorescence stained using TUNEL assay to detect apoptotic cells (D1, 2). Magnification 400 ×. Immunofluorescence stain shows TUNEL-positive cells (green), nuclei (dark blue). Quantification of TUNEL-positive cells was performed and represented in (E). The data is represented as mean ± SE (n = 4–6/group), *P < 0.05, **P < 0.01.

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