Figure 2
From: Activated T cell therapy targeting glioblastoma cancer stem cells
T cell activation with DCs loaded with GSC-eluted peptides. (a) Cell growth curve of activated T cells. 40 U/mL of IL-2 was added to the medium every other day until day 10. (b) Expression of CD4 and CD8 by CD3+ T cells at day 7 and 12. (c) Surface markers of T cells by flow cytometry. (d) ELISpot assay to measure IFN-γ secretion of T cells against DCs pulsed with or without GSC-eluted peptides. T cells without DCs were also measured as comparison. T0: naïve T cells, TNEP12: T cells co-cultured with non-peptide loaded DCs for 12 day, TEP12: T cells co-cultured with eluted peptides-loaded DCs for 12 day. ns, no significant difference.
