Figure 6
From: Activated T cell therapy targeting glioblastoma cancer stem cells
Workflow of producing tumor-associated antigens (TAA)-activated T cells. There are four main steps after isolation of monocytes and naïve T cells from donor’s leukapheresis product by elutriation. Isolated naïve T cells were cryopreserved for later use. Immature DCs were differentiated from monocytes and loaded with TAA (GSC lysate, GSC-eluted peptides or GBM-specific peptide pool) at day 4. TAA-loaded mature DCs were co-cultured with thawed naïve T cells to activate T cells specific for TAA (TAA-ATCs), followed by expansion of TAA-ATCs to obtain enough amount. TAA-unspecific ATCs were also generated by co-culturing naïve T cells with TAA-unloaded DCs. ATCs were cryopreserved at day 7 and 12 for comparison.
