Figure 4 | Scientific Reports

Figure 4

From: Transcriptional activity mediated by β-CATENIN and TCF/LEF family members is completely dispensable for survival and propagation of multiple human colorectal cancer cell lines

Figure 4

WNT/β-CATENIN target gene expression is reduced in β-CATENIN-deficient CRC cells. (a–d) Expression of the WNT/β-CATENIN target genes AXIN2, ASCL2, RNF43, MYC, and TERT was analyzed by qRT-PCR in HT29 (a), HCT116 (b), SW480 (c) and RKO (d) cell clones with biallelic WT and mutant CTNNB1 genes. Transcript levels were normalized to GAPDH, and are displayed as relative expression (rel. expr.). The box plots display aggregated expression data from CTNNB1+/+ and CTNNB1−/− cell clones. Each dot represents a separate measurement with dot color identifying individual clones. For statistical analysis, LMM was performed (n = 3). nd not detectable, ns not significant.

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