Figure 4
Interspecific comparison of IPI response properties of AMMC-B1 neurons. (a) IPI response properties of JO-B and AMMC-B1 neurons in D. melanogaster. Modified from Yamada et al.31. (b) Time traces of AMMC-B1 responses to artificial pulse songs with different IPIs. Responses to songs with 15–55 ms IPIs in D. melanogaster (blue) and D. simulans (green) are shown (See Supplementary Fig. S4 for all IPI data). Sound stimulus was comprised of 20 pulses for each IPI song (shaded gray area). Pulse songs were designed with a conspecific IPF (see Supplementary Fig. S1). Thin and bold lines show time traces of the response in each individual and the average of all individuals, respectively. (c) Normalized peak responses of AMMC-B1 neurons to pulse songs in D. melanogaster (blue) and D. simulans (green). Dots show normalized peak responses in each individual, where dots of the same individuals are connected with lines. (d) Differences from normalized peak responses to pulse song with 25-ms IPI (i.e., ∆responses) in D. melanogaster (blue) and D. simulans (green). *p < 0.05; two-tailed t-test with Bonferroni correction. Crossbars show median and dots represent individual data. (e) Normalized peak responses fitted with Bayesian hierarchical model in D. melanogaster (blue) and D. simulans (green). Solid smooth lines represent fitted curves. Dots show normalized peak responses in each individual. Dots of the same individuals are connected with thin lines. Left: an overview of the fitting curve. Right: magnified view of short IPI range (left panel grey square). Dashed lines represent IPI where response peaks in D. melanogaster (blue) and D. simulans models (green), respectively. (f) Parameter values obtained from the fitted functions of D. melanogaster (blue) and D. simulans (green). Dots and crossbars represent the estimated parameters of fitting curves for each individual and all individuals, respectively. *p < 0.05, ***p < 0.001; Exact Wilcoxon rank sum test. (g) IPI at peak of individual fitting curve (See Supplementary Fig. S4) in D. melanogaster (blue) and D. simulans (green). Dots and crossbars indicate the estimated peak IPI of fitting curves for each individual and all individuals, respectively. ***p < 0.001; Exact Wilcoxon rank sum test.
