Figure 2
Gender-based expression stability analysis and ranking of the 4 reference genes provided by RefFinder tool and different algorithms considered by RefFinder for the NGT group (n = 47) and separately for NGT Males (n = 29) and NGT Females (n = 18). Comprehensive ranking of the reference genes provided by RefFinder for (a) Males, (b) Females; ranking of the reference genes by Delta Ct method41 for (c) Males, (d) Females; BestKeeper40 for (e) Males, (f) Females; NormFinder39 for (g) Males, (h) Females; and GeNorm37,38, based on average stability value (M value) for (i) Males, (j) Females; The most stable genes are on the left side and the least stable genes are arranged on the right side. The stability values, for each gene are indicated as data labels on the bar-graphs.
