Table 2 Descriptive statistics of raw Ct values of the 4 reference genes (ACTB, PPIB, YWHAZ and GAPDH) in PBMCs of the 86 study subjects categorized into normoglycemic controls (NGT, n = 47) and Type 2 diabetic patients (T2DM, n = 39).
Reference genes | All samples (n = 86) | NGT (n = 47) | T2DM (n = 39) | P | |
|---|---|---|---|---|---|
ACTB | Mean ± SD | 21.3 ± 2.6 | 21.0 ± 2.7 | 21.6 ± 2.5 | 0.264 |
Median (Q1, Q3) | 21.3 (18.9, 23.3) | 20.4 (18.8, 22.9) | 21.7 (20.0, 23.5) | ||
Range (Min, Max) | 9.6 (16.9, 26.4) | 9.6 (16.9, 26.4) | 8.9 (17.1, 25.9) | ||
GAPDH | Mean ± SD | 30.0 ± 4.2 | 29.5 ± 4.1 | 30.5 ± 4.4 | 0.315 |
Median (Q1, Q3) | 29.9 (26.7, 32.0) | 29.5 (25.8, 32.3) | 30.4 (27.4, 32) | ||
Range (Min, Max) | 17.1 (22.9, 40.0) | 17.1 (22.9, 40.0) | 17.0 (23, 40.0) | ||
YWHAZ | Mean ± SD | 26.5 ± 3.6 | 26.2 ± 3.5 | 26.8 ± 3.6 | 0.530 |
Median (Q1, Q3) | 26.1 (23.6, 28.5) | 25.6 (23.6, 28.5) | 27.3 (24.2, 28.5) | ||
Range (Min, Max) | 19.6 (20.4, 40.0) | 18.6 (21.4, 40.0) | 19.6 (20.4, 40.0) | ||
PPIB | Mean ± SD | 30.2 ± 4.6 | 30.1 ± 5.1 | 30.4 ± 3.8 | 0.190 |
Median (Q1, Q3) | 29.5 (26.7, 32.6) | 29.0 (26.3, 32.2) | 30.9 (27.6, 32.9) | ||
Range (Min, Max) | 17.4 (22.6, 40.0) | 16.5 (23.5, 40.0) | 17.4 (22.6, 40.0) |
