Figure 1
From: Neuronal glutathione loss leads to neurodegeneration involving gasdermin activation
Neuronal GCLC-deficiency leads to brain atrophy due to neuronal loss. (A) Brain sections from 4-, 8-, 14- and 19-month-old GCLC-KO were stained by H&E. Lower right panel shows a magnified image of a 19-month-old mouse brain. (B) Representative MRI scans of GCLC-KO mouse brains scanned every 2 months from 3 to 11 months of age. (C) Values shown in the graph represent the mean bilateral hippocampal volume calculated from 8–9 scanned MR images (left) and cortical thickness measured from several images (right) ± SEM (n = 4; *p < 0.05, **p < 0.01). (D) Brain sections of 8-month-old GCLC-KO and WT were immunostained for NeuN (green)/cleaved-caspase 3 (red). Right panels were confocal MIP images of cortex (Upper panels) and hippocampal CA1(Lower panels) region. Values shown in the graphs are the fluorescence intensity of NeuN and caspase 3 with the results expressed as the mean relative levels ± SEM (n = 4; *p < 0.05, **p < 0.01).
