Figure 2
From: Neuronal glutathione loss leads to neurodegeneration involving gasdermin activation
GCLC deficiency induces severe neuroinflammation. (A) Brain sections of 5-month-old WT (each left panel) and GCLC-KO (each right panel) were immunostained using Iba1 (left, red) or GFAP (right, Green) antibody and stained with Hoechst (Blue). Upper panels show hippocampal CA1 region, and lower panels show cortical region. Values shown in the graphs represent the mean intensity level ± SEM (n = 3; **p < 0.01, ***p < 0.001). (B,C) Brain sections of 3-,4-,8- and 19-month-old GCLC-KO mice were immunostained with Iba1 (B) or GFAP (C) antibody. (D) Representative images of brain sections from 5-month-old WT and GCLC-KO immunostained with TSPO (Green) and Iba1 (red) antibodies. Upper panels show hippocampal CA1 region, and lower panels show cortical region. Values shown in the graphs represent the mean intensity level ± SEM (n = 3; ***p < 0.001). (E,F) mRNA levels of microglial (E) and astrocyte (F) markers in 4-month-old WT and GCLC-KO were determined by qRT-PCR. Values shown in the graphs represent the mean relative expression level ± SEM (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). (G) Tris–HCl soluble fractions were prepared from the cortices of 4-month-old WT and GCLC-KO. HMGB1 protein levels were determined. Values shown in the graph represent relative expression level of HMGB1 (n = 3; *p < 0.05).
