Figure 2
From: Inhibition of the TRIM24 bromodomain reactivates latent HIV-1
IACS-9571 stimulates HIV-1 expression in primary CD4+ T cells. (A) CD4+ T cells were pre-treated for one hour with vehicle control or 10 μM IACS-9571 and subsequently infected with RGH; flow cytometry was performed 3 days later. (B) The proportion of productively infected CD4+ T cells following 3 days treatment with 10 μM IACS-9571 or vehicle control is shown. Error bars represent standard deviation of 3 measurments. (C) As in Panel B but evaluating the Mean Fluorescence Intensity (MFI) of GFP expression. The GFP MFI was evaluated within the entire population of infected cells (All Infections) or within the population of cells that harbored productive infections (Productive). (D) Cells were treated as in (A,B) and viability was measured. Measurements were performed in duplicate with error bars representing standard deviation.
