Figure 9
From: Inhibition of the TRIM24 bromodomain reactivates latent HIV-1
IACS-9571 activates HIV-1 expression synergistically with PEP005 in CD4+ cells from HIV-1 infected individuals. (A–F) CD4+ PBMCs isolated from HIV-1 patients on ART were treated with DMSO (Ve), 10 µM IACS-9571, 15 nM PEP005, or with 10 µM IACS-9571 and 15 nM PEP005 for 20 h. Following incubation, intracellular RNA was extracted and subjected to RT-PCR analysis with oligos targeting HIV-1 mRNA. The results of 2 or 3 RT-PCR determinations are displayed with standard deviation depicted as error bars.
