Figure 4
From: Mechanism of cystogenesis by Cd79a-driven, conditional mTOR activation in developing mouse nephrons
Localization of Cd79a-Cre activity in developing tubules of red fluorescent protein (RFP) reporter mice. Renal phenotypes of Cd79a-Cre;Tsc1ff.;RFPf/+ mice are shown. The RFP reporter traces cell lineage, and the Cd79a promoter drives Cre recombination. (a) Immunohistochemistry staining of kidney sections at postnatal day (P) 0, the RFP signal is found along the ureteric bud tip and stalk in the superficial nephrogenic cortex (arrows) and in the developing distal tubules of the medulla (arrowheads). (b) At P7, RFP signals extend over the medulla along the collecting ducts (arrowheads), in addition to being expressed in the distal convoluted tubules of the superficial cortex (arrows). (c) Immunofluorescence staining of kidney sections at P7 with an anti-RPF antibody as well as markers for proximal tubules (LTL, green) and distal tubules and collecting ducts (DBA, red). RFP signals mostly colocalized with DBA, indicating that the Cd79a promoter is active in ureteric buds and their progenitors in developing distal nephrons. Inset: Dashed box areas are magnified.
