Figure 4

Sinococuline effectively reduces dengue virus load in several organs of severely DENV-infected AG129 mice. Group of AG129 (n = 3) inoculated with IC and treated with different doses of Sinococuline (i.p), viz., 0.5 mg/kg/day, 1.0 mg/kg/day and 2.0 mg/kg/day, in a BID manner for 4 days. On day-4 (post completion of dosing), mice were euthanised by CO₂ inhalation followed by cervical dislocation, and extensively perfused with 1× PBS. The vital organs were collected (50 mg each organ) in 500 µl of RNA stabilising solution. Virus load in different organs (small intestine, large intestine, lung, liver, kidney and spleen) were estimated by SYBR green-based RT-qPCR. Data were normalised from the only Sinococuline control group (uninfected mice who received highest dose of Sinococuline i.e., 2.0 mg/kg/day; BID) which was considered as the baseline. */** indicates statistically significant and ns: not significant across all the panels while data were analysed by using Two-way ANOVA with GraphPad Prism software.