Figure 2
Phagocytosis of S. aureus by macrophages over time. (a–b) Percentage (%) of RAW264.7 macrophages with different numbers of internalised bacteria after incubation with S. aureus for 0.5 h (white bars), 1 h (shaded bars), 1.5 h (black bars) and 2.5 h (grey bars). Bars represent mean percentage ± SEM (n = 3), dotted horizontal lines show 1% and 5%. (a) S. aureus MOI 5; (b) S. aureus MOI 50. (c–d), representative images of RAW264.7 macrophages (red) with internalised bacteria after incubation with GFP S. aureus (green) for 2.5 h: (c) MOI 5 S. aureus, (d) MOI 50 S. aureus (scale bars 50 µm).
