Figure 2 | Scientific Reports

Figure 2

From: Myosin light chain phosphorylation exhibits a gradient across the wall of cerebellar arteries under sustained ex vivo vascular tone

Figure 2

The pattern of MLC-p and SMA-α-actin staining is not due to limited antibody diffusion across the vessel wall. (A) Immuno-staining of desmin (green) and GFAP (green) in the vessel wall of rat SCA. Vessel was fixed while maintaining a stable myogenic tone at 70 mmHg. (B) Triple staining of myosin heavy chain type A (MHA, red), MLC-p (green) and phalloidin (yellow) in the vessel wall of SCA. (C) Triple-staining of pan-actin (green), SM-α-actin (purple) and phalloidin (yellow) in the vessel wall of SCA. a: depicts adventitia side of vessel wall; l: depicts intra-luminal side of vessel wall.

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