Figure 2

Effect of the (P)RR Ab on Wnt signaling and stemness markers. Using Cells were treated with the (P)RR Ab at 400 µg/mL. (A) Representative western blot images showing that treatment with the (P)RR Ab reduced the activity of the Wnt/β-catenin signaling pathway [active β-catenin and c-myc] and expression of stemness markers (SOX2 and Oct3/4) in U251MG, U87MG and MGG23 cells. Original blots are presented in Supplementary Fig. S5. (B) Relative protein levels measured using western blotting, quantified and normalized to β-actin levels. C control, A antibody.