Table 1 Summary of proliferation, senescence (β-galactosidase activity), flow cytometry and qPCR (gene expression) analyses performed in wild-type (wt) and Ink4a/Arf^−/− tenocytes.

		wt		Ink4a/Arf^−/− low passage			Ink4a/Arf^−/− intermediate passage			Ink4a/Arf^−/− high passage
		Mean	s.d	Mean	s.d	p value	Mean	s.d	p value	Mean	s.d	p value
Proliferation (number of cells)	24 h	3159	561.1	4056	654.9	0.96	3201	651.8	0.99	1807	626.6	0.88
	48 h	5032	841.9	6338	1407	0.89	7872	1741	0.41	6457	868	0.86
	72 h	4811	1109	7186	1448	0.56	16,342	6600	< 0.0001	16,772	1259	< 0.0001
	96 h	5259	1107	7196	1152	0.71	22,917	9525	< 0.0001	25,785	3153	< 0.0001
Cellular senescence (β galactosidase activity) (Fluorescence a.u)		528.2	122.8	260	42.72	0.008	114.8	31.87	0.0005	218	22.19	0.003
Flow cytometry (% of living cells)	CD29	99.9	0.01	99.93	0.06	0.99	99.56	0.5467	0.29	99.98	0.02082	0.99
	CD105	38.8	35.09	37.6	8.99	0.99	10.05	17.07	0.36	0.2967	0.4712	0.16
	CD90	93.7	1.29	95.28	1,776	0.99	91.95	8,828	0.99	25.79	35.89	0.009
	CD44	98.86	1.54	99.24	0.31	0.95	98.61	0.4388	0.98	99.5	0.5746	0.79
Gene expression 2D (normalized mRNA level)	Collagen 1	8.16	4.6	5.5	5.2	0.9	5.7	5.6	0.9	4.5	4.7	0.8
	Collagen 3	2.2	1.4	0.4	0.3	0.47	1.9	1.5	0.99	1.8	1.7	0.99
	Collagen 5	0.2	0.1	0.04	0.02	0.36	0.09	0.11	0.72	0.09	0.12	0.7
	Tenomodulin	0.08	0.008	0.02	0.03	0.22	0.03	0.04	0.3	0.03	0.04	0.27
	Tenascin C	0.13	0.06	0.03	0.01	0.25	0.07	0.05	0.6	0.07	0.07	0.6
	Scleraxis	0.01	0.01	0.01	0.004	0.9	0.01	0.003	0.8	0.02	0.01	0.99

Results are presented as mean ± s.d. Ink4a/Arf^−/− cells (in low, intermediate, and high passages) were compared to wt cells using an ANOVA with Tukey’s multiple comparisons test, and the p-value is shown for each evaluation. (a.u.—arbitrary units).

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