Table 2 qPCR analysis of tendon markers expression in wild-type (wt) and Ink4a/Arf−/− tenocytes in 2D and 3D culture.

From: Immortalized murine tenocyte cells: a novel and innovative tool for tendon research

2D wt (low passage) (mean ± s.d)

3D wt (low passage) (mean ± s.d)

Significance wt 3D\2D

2D Ink4a/Arf−/− (all passages) (mean ± s.d)

3D Ink4a/Arf−/− (all passages) (mean ± s.d)

Significance Ink4a/Arf−/− 3D\2D

8.2 ± 4.7

87.6 ± 38.3

****

5.4 ± 5.1

30.4 ± 17.2

**

2.2 ± 1.4

10.5 ± 7.6

*

1.4 ± 1.7

7.6 ± 7.0

*

0.2 ± 0.1

1.01 ± 0.46

***

0.1 ± 0.1

0.6 ± 0.5

**

0.1 ± 0.001

0.05 ± 0.1

NS

0.03 ± 0.03

0.04 ± 0.08

NS

0.1 ± 0.1

1.4 ± 0.7

***

0.06 ± 0.06

0.63 ± 0.68

*

0.01 ± 0.01

0.01 ± 0.01

NS

0.01 ± 0.01

0.01 ± 0.003

NS

  1. Values are shown as mean ± s.d. normalized mRNA levels. 3D tendon-like constructs and 2D monolayer culture were compared for each cell type using a t-test, and the p-value is shown for each comparison. For the Ink4a/Arf−/− tenocytes, cells from low, intermediate, and high passage were combined.
  2. Significance between 3 and 2D as follows- *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001. NS not significant.
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