Table 4 Used labeling parameters in TestSTORM.
From: Machine learning framework to segment sarcomeric structures in SMLM data
Labelling parameters | ||
|---|---|---|
Double-line objects | Noise | |
Emission WL (nm) | 665 | 665 |
Char. ON time (s) | 0.03 | 0.05 |
Char. OFF time (s) | 50 | 42 |
Bleaching constant (s) | 1700 | 1700 |
Emitted photon/s | 80,000 | 100,000 |
Mean bonding angle (\(^\circ\)) | 0 | 0 |
SD of bonding angle (\(^\circ\)) | 30 | 30 |
Mean N of labels/epitope | 5, 6 | 4, 6 |
Var. N of labels/epitope | 2 | 0, 1, 2 |
Length of linkers (nm) | 25, 30 | 10, 15, 30 |
Non-spec. l. dens. (\(1/\mu \textrm{m}^3\)) | 0 | 0, 3 |
