Table 5 Used acquisition parameters in TestSTORM.
From: Machine learning framework to segment sarcomeric structures in SMLM data
Acquisition parameters | |
|---|---|
Number of frames | 15,000–20,000 |
Frame rate (1/s) | 20 |
Exp. time (s) | 0.02 |
Pixel size (nm) | 160 |
Av. BG level | 200 |
RI of immersion m. | 1.518 |
RI of sample m. | 1.331 |
Numerical aperture | 1.4 |
Electron/count | 21.5 |
Pre-amplification | 2.5 |
Actual EM gain | 90 |
Quantum efficiency | 0.9 |
