Table 2 Summary of technical parameters for C6A6.
TECHNICAL VALIDATION | RESULTS (Accepted recovery: 100 ± 20%) |
|---|---|
IC50 | 3.86 ng/mL |
Measurement range (LLMR-ULMR) | 0.60–13.00 ng/mL |
Inter-assay variation, mean (range) | 5% (7.31–18.75) |
Intra-assay variation, mean (range) | 12% (2.4–12.05) |
Dilution recovery of human serum, mean (range) | 105% (85.4–120.4) |
Spiking recovery (selection peptide in serum), mean (range) | 104% (104–109) |
Analyte stability and recovery, 24 h at 4 °C/20 °C , mean (range) | 93% (85.9–96.8)/81% (66.8–90.6) |
Freeze–thaw stability, five cycles, mean (range) | 97% (72.6–103.5) |
Hemoglobin interference (low/high), mean (range) | 102% (87.1–121.3) / 85% (76.2–98.0) |
Lipemia interference (low/high), mean (range) | 105% (82.6–120.5) / 107% (80.8–130.4) |
Biotin interference | < 10 ng/mL |
