Figure 3
From: Epithelial cell-derived cytokine TSLP activates regulatory T cells by enhancing fatty acid uptake
TSLP signaling enhances activation marker expression during experimental colitis in a competitive setting. (A–G) Adoptive transfer model of colitis. n = 7 from 2 independent experiments. (A) The same number of congenically marked naïve CD4+ T cells of Foxp3YFP-Cre mice and Foxp3YFP-CreTSLPRf/f mice were injected into RAG2−/− mice. (B) Bodyweight changes of the mice. (C) Representative FACS plots of Foxp3 vs. CD45.1 (gated on CD4+ T cells) in colonic LP, mLN, and spleen and cumulative data of CD4+ T cells expressing Foxp3. (D) CD44 expression on Treg cells in colonic LP, mLN, and spleen. Representative histograms of CD44 expression (gated on Treg cells) and their cumulative data. (E) CD44 expression on non-Treg cells in colonic LP, mLN, and spleen. Representative histograms of CD44 expression (gated on non-Treg cells) and their cumulative data. (F) Cell size (forward scatter [FSC]) of Treg cells of Foxp3YFP-Cre mice and Foxp3YFP-CreTSLPRf/f mice in the adoptive transfer model of colitis. (G) Cell size (FSC) of non-Treg cells of Foxp3YFP-Cre mice and Foxp3YFP-CreTSLPRf/f mice in the adoptive transfer model of colitis. *P < 0.05, and **P < 0.01 as determined by paired t-test.
