Figure 4
From: Epithelial cell-derived cytokine TSLP activates regulatory T cells by enhancing fatty acid uptake
TSLP increases the size of Treg cells. (A–D) Cell size and cell division by TSLP. Naïve CD4+ T cells labeled with CellTrace Violet (CTV) were cultured under iTreg conditions for 3 days in the presence or absence of TSLP. The expression of Foxp3 defines Treg cells. Non-Treg cells are Foxp3− cells in the same samples. n = 3 from 2 independent experiments. (A) Cell size determined by FSC of PBS- or TSLP-treated CD4+ T cells. (B) Left, representative histograms of CTV dilution. Right, cumulative data. (C) Apoptosis analysis. In vitro-induced Treg cells were stained with Annexin-V and 7-AAD. Left, representative FACS plots (gated on Treg cells). Right, cumulative data. n = 3 from 2 independent experiments. (D) Treg cell activation independent of cell proliferation. CTV-labeled cells cultured in the presence or absence of TSLP. Gated were the cells which did not proliferate and analyzed for the expression of CD44. n = 5 from 2 independent experiments. *P < 0.05 as determined by unpaired t-test.
