Table 1 Summary of the performance of LAMP targeting the B. bovis cytb gene compared to PCR-AGE methods.

Method	Visualization		External PCR (Romero-Salas et al.⁴¹)			McNemar’s Test	Cohen’s	Sensitivity	Specificity
			Pos	Neg	Total	p—value	kappa	(%)	(%)
Internal PCR	UV light	Pos	33	0	33
control	(Gel	Neg	0	66	66	1	1	100	100
	electrophoresis)	Total	33	66	99
	Neon light	Pos	6	0	6
		Neg	27	66	93	0.00***	0.229	18.2	100
		Total	33	66	99
	LED light	Pos	28	1	29
		Neg	3	67	70	0.625	0.904	90.3	98.5
LAMP		Total	31	68	99
	UV light	Pos	32	0	32
		Neg	1	66	68	1	0.954	97	100
		Total	33	66	99
	UV light	Pos	32	0	32
	(Gel	Neg	1	66	68	1	0.954	97	100
	electrophoresis)	Total	33	66	99

The bold number with 3 asterisks (***) represents the statistical significance of McNemar’s test as a p-value ≤ 0.05. Cohen’s kappa coefficient; < 0 = no agreement, 0–0.20 = slight, 0.21–0.40 = fair, 0.41–0.60 = moderate, 0.61–0.80 = substantial, 0.81–1 = almost perfect agreement.
Pos. Positive, Neg. Negative, UV Ultraviolet, LED Light- Emitting Diode.

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