Figure 2

Phylogenetic tree built using 68 full reference genomes of CoV species and one reference genome of Edwardsiella virus pEt-SU (indicated by the NC-code from the NCBI RefSeq database). The rectangular heatmap represents the number of mismatches for forwards (F) and reverse (R) primers from Vijgen et al.²⁹ (PrimeA), Lelli et al.¹³ (PrimeB), and Watanabe et al.³⁰ (PrimeC) during in silico PCR testing.