Table 1 Analytical parameters of GC/MS analysis of SCFAs.

From: Effect of in vitro cultivation on human gut microbiota composition using 16S rDNA amplicon sequencing and metabolomics approach

SCFAs

tR (min)

m/z

R2

Linearity range (µg/ml)

LOD

LOQ

Rec (%)

RSD (%)

Acetic acid

5.247

87.00

0.9984

125–94,000

9.37

31.24

117

1.1381

Propionic acid

6.203

75.00

0.9978

125–4000

20.68

68.93

118

0.8906

Isobutyric acid

8.121

89.00

0.9985

125–4000

4.12

13.72

102

0.5987

Butyric acid

6.281

89.00

0.9998

125–4000

1.96

6.53

97

0.6954

Valeric acid

11.524

85.00

0.9998

125–4000

0.75

2.50

99

0.2562

Isovaleric acid

9.401

85.00

0.9997

125–4000

3.14

10.48

84

0.5938

  1. tR retention time, m/z mass to charge ratio, R2 coefficient of determination of linear regression, LOD limit of detection, LOQ limit of quantification, Rec recovery rate, RSD relative standard deviation.
Back to article page