Figure 7
From: Agricultural dust derived bacterial extracellular vesicle mediated inflammation is attenuated by DHA
DHA-rich PUFA ratios dampened DE-derived EV-induced inflammatory cytokine release, but augmented AREG release during recovery. HBEC were stimulated with concentrated DE-derived EVs (50 × 109/mL) for 2 h, EVs were then removed, and cultures were allowed to recover in the presence of PUFA ratios for 22 h. Release of EV-mediated proinflammatory cytokines IL-6 and IL-8 was attenuated, and pro-repair AREG was augmented by treatment with the DHA-rich (1:1) PUFA ratio and by DHA alone during the recovery phase. Data shown are means of 4 independent experiments with 24 technical replicates per condition. *p < 0.05, **p < 0.01, ***p < 0.0001 (ANOVA, Tukey’s posttest).
