Figure 1

Experiment. Step 1: Short synthetic DNA strands with a central random hexamer (black) flanked by a tag sequence (brown) and spacer random nucleotide (blue) are synthesized to form a naïve sequence library. Step 2: The DNA strands in a cuvette irradiated with UV laser light at 266 nm to induce photolesions. Step 3: The irradiated sample is split into a number of samples with defined irradiation doses. Subsequent library preparation for high throughput sequencing selects and amplifies only intact sequences without UV lesions for next generation sequencing (Step 4). Step 5: Analysis of the data, normalization and correction yields the abundance of sub-mers up to hexamer length for subsequent analysis of the sequence dependent damage process.