Table 1 Mouse primer Sequences for QPCR.
From: CD47 antisense oligonucleotide treatment attenuates obesity and its-associated metabolic dysfunction
Gene | Primer sequence | Genes | Primer sequence |
|---|---|---|---|
FASN | 5’-TCCTGGAACGAGAACACGATCT-3’ 5’-GAGACGTGTCACTCCTGGACTTG-3’ | SCD-1 | 5’-TTCTTGCGATACACTCTGGTGC-3’ 5’-CGGGATTGAATGTTCTTGTCGT-3’ |
ACC | 5’-CCCAGCAGAATAAAGCTACTTTGG-3’ 5’-TCCTTTTGTGCAACTAGGAACGT-3’ | SREBP1c | 5’-GGAGCCATGGATTGCACATT-3’ 5’-ACAAGGGTGCAGGTGTCACC-3’ |
HMGCR | 5’-TGCACGGATCGTGAAGACA-3’ 5’-GTCTCTCCATCAGTTTCTGAACCA-3’ | HMGCR | 5’-TGCACGGATCGTGAAGACA-3’ 5’-GTCTCTCCATCAGTTTCTGAACCA-3’ |
F4/80 | 5’-CTTTGGCTATGGGCTTCCAGTC-3’ 5’-GCAAGGAGGACAGAGTTTATCGTG-3’ | IL-1β | 5’-TGGAGAGTGTGGATCCCAAGCAAT-3’ 5’-TGTCCTGACCACTGTTGTTTCCCA-3’ |
TNFα | 5’-AGCCGATGGGTTGTACCT-3’ 5’-TGAGTTGGTCCCCCTTCT-3’ | CD4 | 5’-TGGCTCAGCTCAACAATAC-3’ 5’-GGCAGCAGCGAAGAATAA-3’ |
CD8 | 5’-AAGAAAATGGACGCCGAACTT-3’ 5’-AAGCCATATAGACAACGAAGGTG-3’ | NOS2 | 5’-AAGAGGAGCAACTACTG-3’ 5’-GCTCTGTTGAGGTCTAA-3’ |
Arg1 | 5’-CTCCAAGCCAAAGTCCTTAGAG-3’ 5’-AGGAGCTGTCATTAGGGACATC-3’ | UCP1 | 5’-ACTGCCACACCTCCAGTCATT-3’ 5’-CTTTGCCTCACTCAGGATTGG-3’ |
UCP2 | 5’-GGCTGGTGGTGGTCGGAGAT-3’ 5’-CCGAAGGCAGAAGTGAAGTG-3’ | UCP3 | 5’-ACCTGGACTGCATGGTAAGG-3’ 5’-GAGAGCAGGAGGAAGTGTGG-3’ |
CPT1α | 5’-CTCTATGTGGTGTCCAAG-3’ 5’-CACAGGACACATAGTCAG-3’ | CPT1β | 5’-ACCTGAGCTGTGCTGAATAAA-3’ 5’-ACAGGAGACGGACACAGATA-3’ |
CD36 | 5’-TTGTACCTGGGAGTTGGCGAGAAA-3’ 5’-ACAGTTCCGATCACAGCCCATTCT-3’ | PGC-1α | 5’-CTGCATGAGTGTGTGCTGTG-3’ 5’-CAAATATGTTCGCAGGCTCA-3’ |
CD31 | 5’-TGCAGGCATCGGCAAAG-3’ 5’-GCATTTCGCACACCTGGAT-3’ | COX1 | 5’-CGTAACTGCCCATGCTTT-3’ 5’-CTGCTCCTGCTTCTACTATTG-3’ |
ATGL | 5’-AACACCAGCATCCAGTTCAA-3’ 5’-GGTTCAGTAGGCCATTCCTC-3’ | HSL | 5’-GGCTCACAGTTACCATCTCACC-3’ 5’- GAGTACCTTGCTGTCCTGTCC-3’ |
β-Actin | 5’-GGCTGTATTCCCCTCCATCG-3’ 5’-CCAGTTGGTAACAATGCCATGT-3’ | mtDNA | 5’-CCGCAAGGGAAAGATGAAAGAC-3’ 5’-TCGTTTGGTTTCGGGGTTTC-3’ |
nDAN | 5’-GCCAGCCTCTCCTGATTTTAGTGT-3’ 5’-GGGAACACAAAAGACCTCTTCTGG-3’ |
