Figure 2 | Scientific Reports

Figure 2

From: Molecular mechanism of valine and its metabolite in improving triglyceride synthesis of porcine intestinal epithelial cells

Figure 2

Valine promoted triglyceride synthesis in IPEC-J2 cells via 3-HIB. Cells were incubated in 0.1 mM valine in medium (Con) and 0.9 mM valine in medium (Val). The cells were transfected with 3-HIB synthase 3-hydroxyisobutyryl-CoA hydrolase HIBCH-siRNAs (siHIBCH) LipofectamineTM 3000. Culture medium collection, total RNA extraction, and fat drop staining was performed after 48 h. (A) 3-HIB concentration in medium with valine treatment; (B) 3-HIB metabolism-related gene mRNA expression; (C) Gene knockdown fficiency of candidate siRNAs in IPEC-J2 cells; (D,E) 3-HIB and TG concentration in the medium and cells after HIBCH silencing; (F) Immunofluorescent staining of lipid droplets. Scale bar represents 50 μm. 3-HIB: 3-hydroxyisobutyrate; HIBCH: hydroxyisobutyryl-CoA deacylase; TG: Triglyceride. All data with error bars represent the mean ± standard error of mean. ∗ P < 0.05.

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