Figure 3
Oxidative stress is reduced in FECD cells when exposed to exogenous mitochondria. (A) A marker of oxidative stress (CM-H2DCFDA; green) was used in conjunction with a mitochondrial mass marker (mitotracker; red) in FECD explants exposed to exogenous mitochondria or not (Control). (B) CM-H2DCFDA signal per cell is significantly reduced in FECD explants that received exogenous mitochondria, compared to control explants. Scale bar = 40 µm. Experiments were performed on 4 explants from 4 FECD patients (Total 41 microscope fields analyzed). *p < 0.005.
