Figure 2 | Scientific Reports

Figure 2

From: Three-dimensional visualization of neural networks inside bone by Osteo-DISCO protocol and alteration of bone remodeling by surgical nerve ablation

Figure 2

The newly established optical bone clearing (Osteo-DISCO) method reveals the 3D structure of the neurovascular network inside bone. (A) Visualized nerves inside bones of Sox10-venus mice (tibia: the whole and magnified midshaft, forearm: radius and ulna, jaw, and femur). White arrowhead: Specific entry site of Sox10-positive nerves. Scale bar: 500 µm. (B) Venus fluorescence of Sox10-positive nerves was preserved even 18 months after clearing. Scale bar: 500 µm. (C) Visualized nerves and blood vessels inside bones of Sox10-Venus; Flt1-tdsRed mice (tibia and jaw). Scale bar: 500 µm. (D) Blood vessels entering bone tissue in Flt1-tdsRed mice. Yellow arrowheads: entry sites of Flt1-positive blood vessels.

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