Table 6 Primer names and sequences, target size and PCR conditions for enterotoxin genes detection58.
From: Bacillus subtilis isolates from camel milk as probiotic candidates
Primers | Target size (bp) | Sequences (5′–3′) | Reaction conditions |
|---|---|---|---|
hblA F hblA R | 1154 | AAGCAATGGAATACAATGGG AGAATCTAAATCATGCCACTGC | 94 °C,2 min/(94 °C,60 s*56 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
hblC F hblC R | 740 | GATACCAATGTGGCAACTGC TTGAGACTGCTCGCTAGTTG | 94 °C,2 min/(94 °C,60 s*58 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
hblD F hblD R | 829 | ACCGGTAACACTATTCATGC GAGTCCATATGCTTAGATGC | 94 °C,2 min/(94 °C,60 s*58 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
nheA F nheA R | 499 | TACGCTAAGGAGGGGCA GTTTTTATTGCTTCATCGGCT | 94 °C,2 min /(94 °C,60 s*56 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
nheB F nheB R | 769 | CTATCAGCACTTATGGCAG ACTCCTAGCGGTGTTCC | 94 °C,2 min/(94 °C,60 s*54 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
nheC F nheC R | 581 | CGGTAGTGATTGCTGGG CAGCATTCGTACTTGCCAA | 94 °C,2 min/(94 °C,60 s*58 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
