Figure 1
From: Molecular testing devices for on-site detection of E. coli in water samples
Exploded view of the device components. The buffer unit at the top contains four reservoirs and an opening. These 4 reservoirs are for the lysis buffer, binding buffer, and 2 wash buffers. The opening at the bottom of each well is blocked by a stainless-steel ball to prevent the reagents from flowing down until it is desired. The mixing unit in the middle is in a funnel shape to enhance mixing and make the reagents and sample pass through the paper in the detection unit, which is inserted by the protrusion (➇) at the bottom of the mixing unit. The mixing unit has a pin that pushes the ball up, opening the valve and allowing the reagents to go down when the buffer unit is rotated to align the pin with the ball. Finally, the waste container in the bottom serves 2 purposes, (1) collecting the waste, and (2) if needed, accelerating the filtration process by connecting a vacuum or a suction mechanism to it.
