Figure 4

Experimental group at 8 weeks observational period (A) Prevalence of bone formation all over the defect. Intercommunicating thick trabeculae are seen enclosing rich blood supply (BV). Well-organized PDL structure is noted H&E, 40×. (B) The organized structure of the regenerating PDL and neighboring structures, H&E, 100X. (C) Denser bone formation in the defect center with enclosing remanent of the dissolving and restricted ß-TCP, H&E, 100×. (D) Longitudinal section of the regenerating periodontium in one of the defects. Well-organized PDL fibers with pronounced blood supply is seen. The alveolar bone border exhibits histological features of remodeling and regeneration, H&E, 100×. (E) Higher magnification of inset 1 in Fig. 4Grevealing the rich blood supply adjacent to the regenerating bone and the adjoining noticeable proliferation of osteoprogenitor cells and osteoblasts H&E, 400×. (F) Higher magnification of inset 2 in Fig. 4G revealing the fiber and cell density in the regenerating PDL with proper and typical fiber direction towards cementum. Cementoblasts are seen between the inserting fibers in close association with a layer of forming cementoid, H&E, 400×. (G) Insertion of the PDL fiber bundles in the regenerating alveolar bone while enclosing osteoblasts among them (H) Voluminous cementoblasts (arrows) resting on a layer of cementoid and accommodating the inserting Sharpey’ fibers. In the last 2 images note the cell density in the ligament and the blood supply (BV), Trichrome stain, 400×.